REPOLARISATION ASSAYS

VENTRICULAR MYOCYTES

Protocol conditions

• Action potential measurements made in single ventricular myocytes


• Stability of APD under control conditions in single ventricular myocytes

Effects of standard comparator compounds on APD

• Sotalol


• Dofetilide

Effects of compounds which alter action potential configuration

• Terfenadine


• Sparfloxacin


• Cisapride


• Haloperidol

Effects of compounds which shorten APD

• Nifedipine


• Verapamil

DELAYED RECTIFIER CURRENT I_K ASSAYS

hERG CHANNEL TAIL CURRENT ASSAYS

Protocol conditions

• Measurement of hERG channel tail current amplitude
  

Effects of comparator compounds on hERG channel tail current

• Dofetilide


• E-4031

I_Kr ASSAYS
VENTRICULAR MYOCYTES
WHOLE-CELL PATCH-CLAMP CONDITIONS

Protocol conditions

• Measurements of I_Kr in single ventricular myocytes
  

Effects of comparator compounds on I_Kr

• Dofetilide


• E-4031

I_Kr ASSAYS
VENTRICULAR MYOCYTES
SWITCH-CLAMP CONDITIONS

Protocol conditions

• Measurements of I_Kin single ventricular myocytes
  

Effects of comparator compounds on I_Kr

• Dofetilide


• Sotalol

LANGENDORFF WHOLE HEART ASSAYS

Protocol conditions

• Measurement of mechanical and electrical activity in a Langendorff whole heart preparation

L-TYPE CALCIUM CURRENT ASSAYS

Protocol conditions

• Measurements of peak L-type calcium current in single ventricular myocytes

Effects of comparator compounds on I_Ca

• Nifedipine

GAP JUNCTIONAL CONDUCTANCE ASSAYS

Protocol conditions

• Measurements of gap junctional current in ventricular myocyte cell pairs

Effects of gap junction uncouplers

• Hepatanol

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