ION CHANNEL ASSAYS

SINGLE VENTRICULAR MYOCYTES

EFFECTS OF NIFEDIPINE ON L-TYPE CALCIUM CURRENT (ICa)

Figure 1 Effects of  nifedipine on peak L-type calcium current ICa

Figure 1 shows an example of a typical ICa recorded by scientists at OCP   recorded under our standard conditions for the measurement of ICa in single left ventricular myocytes using the whole-cell patch-clamp technique.
The time course for the effect of nifedipine is shown on the left panel and the bar above the records denotes application of nifedipine.  Representative calcium current records (taken from the same single cell) recorded in the absence (control, black) and presence of nifedipine (red and denoted by arrow) is shown in the right panel.
Nifedipine (300 nM, application denoted by the bar, note the dotted line denotes zero current level) caused complete block of ICa.
Nifedipine inhibited ICa with an IC50 of 5.4 nM.

 
 

Figure 2   Log(concentration)-response curve for the effect of nifedipine on ICa measured in guinea-pig myocytes

 
 
 
   
  All data 㯰yright to OCP Ltd. 2006. All Rights Reserved. Design by OCP