ION CHANNEL ASSAYS

VENTRICULAR MYOCYTE CELL PAIRS

EFFECTS OF HEPTANOL ON JUNCTIONAL RESISTANCE MEASURED IN VENTRICULAR MYOCYTE CELL PAIRS

Figure 1  Effect of heptanol on junctional conductance measured in isolated ventricular myocyte cell pairs.

Figure 1 shows the effects of the uncoupling agent heptanol on junctional current amplitude.  A repetitive step hyperpolarisation to -50 mV (0.2 Hz) is used to monitor the stability of the amplitude of IJ.  After 2 minutes control recording the perfusate is switched to one containing heptanol (as denoted by the black bar). 
Heptanol which is a known uncoupler of cardiac gap junctions causes an immediate and complete block of IJ amplitude.  On wash off of heptanol; there was complete recovery of IJ.

 

 

 
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